The CelEct Virus Universal Probe One-step kit is developed for fast and reproducible detection of viral RNA targets using probe-based real time one-step PCR. It can achieve sensitivity down to 4 copy inputs of RNA target and is compatible with probes based on various principles, including TaqMan®, Scorpions® and molecular beacon probes.
The 4X concentrated mix achieves ultra-low sensitivity by increasing the sample input volume and is ideal for high throughput assays with small reaction volumes. In addition to the advanced buffer composition, an RNase inhibitor protects viral RNA from degradation by RNases and complements the thermostable CelScript Reverse Transcriptase.
An antibody-mediated hot-start polymerase limits the formation of primer dimers and other non-specific products leading to efficient amplification of multiple targets, even in the presence of common inhibitors found in clinical samples.
The qPCR mix is supplied with a separate tube of 50μM ROX dye that may be used with instruments requiring ROX as a passive reference dye. CelProbe Universal qPCR mix is suitable for use on all real time PCR cyclers.
For research use only. Not for use in diagnostic procedures.