CelEct Virus Universal Probe One-step kit

qPCR One-step Probe mix for reliable, accurate detection of viral RNA targets
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Highlights

  • Validated for qualitative detection of SARS-CoV-2 (COVID-19)
  • Ultra-low sensitivity to low copy number templates
  • 4X concentrated buffer enables more sample input volume
  • Contains the thermostable CelScript reverse transcriptase and novel Rnase inhibitor
  • Yields early Ct values with wide dynamic detection range
  • Compatible with TaqMan®, Scorpions® and molecular beacon probes
  • Enhanced specificity supported by a hot-start enzyme
  • Compatible with all qPCR cyclers

Description

The CelEct Virus Universal Probe One-step kit is developed for fast and reproducible detection of viral RNA targets using probe-based real time one-step PCR.  It can achieve sensitivity down to 4 copy inputs of RNA target and is compatible with probes based on various principles, including TaqMan®, Scorpions® and molecular beacon probes.

The 4X concentrated mix achieves ultra-low sensitivity by increasing the sample input volume and is ideal for high throughput assays with small reaction volumes.  In addition to the advanced buffer composition, an RNase inhibitor protects viral RNA from degradation by RNases and complements the thermostable CelScript Reverse Transcriptase.

An antibody-mediated hot-start polymerase limits the formation of primer dimers and other non-specific products leading to efficient amplification of multiple targets, even in the presence of common inhibitors found in clinical samples.

The qPCR mix is supplied with a separate tube of 50μM ROX dye that may be used with instruments requiring ROX as a passive reference dye.  CelProbe Universal qPCR mix is suitable for use on all real time PCR cyclers.

 

Use restrictions

For research use only.  Not for use in diagnostic procedures.

Applications

  • Qualitative detection of SARS-CoV-2/COVID-19
  • Efficient amplification in single- and multiplex reactions
  • Ultra-sensitive detection of RNA viruses
  • Diagnostic qPCR assays
  • Detection of low copy number targets

Specification

Detail
Instrument compatibility For use with all real time PCR instruments (supplied with a separate tube of rox dye)
Amplification detection TaqMan®, Scorpions® and molecular beacon probes
Format Ready-to-use one-step mix (4X)
Polymerase CelTaq HS DNA polymerase
Reverse Transcriptase CelScript Reverse Transcriptase
Increased specificity Hot-start modification
Cycling speed Standard and fast modes
Shelf life 12 months (if stored correctly)

 

Ordering Info

Cat no. Pack size 4X CelEct Virus Universal Probe One-step mix (no-ROX) 20X CelScript Reverse Transcriptase ROX Additive (50μM)
CM3711-0200 200 rxns 1 x 1ml 1 x 0.2ml 1 x 0.2ml
CM3711-0600 600 rxns 3 x 1ml 1 x 0.6ml 1 x 0.2ml
CM3711-0600 1000 rxns 1 x 5ml 1 x 1ml 1 x 0.2ml

Documents

CelEct Virus Universal Probe One-step kit Product Brochure

CelEct Virus Universal Probe One-step kit Product Manual

CelEct Virus Universal Probe One-step kit Product Manual -mobile

CelEct Virus Universal Probe One-step kit MSDS

Storage

Store the kit at -20°C upon arrival.  Full enzyme activity is retained for 12 months when stored at the recommended conditions and can endure up to 30 freeze/thaw cycles. Limit direct light exposure for extended periods.

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