CelGREEN Universal qPCR mix combines an enhanced buffer system containing a proprietary intercalating dye with an advanced enzyme to result in fast, highly specific PCR with market-leading sensitivity.
The antibody-mediated hot start polymerase limits the formation of primer dimers and generates highly specific products from extremely low copy number templates. The proprietary intercalating dye that enables amplicon quantification does not affect the efficiency of the PCR (as reported for other commonly used dyes). The mix contains all components required for sensitive, reproducible qPCR (expect the template and primer pair) with little or no optimization required.
The ready-to-use real time PCR mix performs well on difficult templates (rich n GC/AT content) and gives reproducible, robust amplification of DNA, cDNA and viral DNA targets.
The Universal qPCR mix is supplied with a separate tube of 50μM ROX dye that may be used with instruments requiring ROX as a passive reference dye. CelGREEN Universal qPCR mix is suitable for use on all real time PCR cyclers.
For research use only. Not for use in diagnostic procedures.