CelTaq DNA Polymerase

DNA Polymerase for routine PCR - everyday
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Cat no. CM2211 Category Feature

Highlights

  • Enhanced sensitivity, speed and yield
  • Efficient amplification of up to 6kb amplicons
  • Superior performance on high GC/AT templates
  • Balanced salt solution with proprietary enhancers and stabilizers eliminates the need for buffer optimization
  • Use with current PCR conditions (compatible with standard and fast cycling)

Description

CelTaq DNA Polymerase is a reliable, cost-effective solution for everyday PCR.  This robust enzyme tolerates PCR inhibitors and gives consistent amplification of difficult templates (egg. mammalian genomic DNA that is rich in GC and AT content). A 12-step purification process eliminates residual host DNA resulting in ultra-low background, while effectively amplifying targets present in low copy numbers.

The enhanced 5x buffer system contains 5mM dNTPs, 15mM MgCl2, stabilizers and enhancers and does not require additional optimization.  The system produces high yields under standard and fast cycling conditions to efficiently amplify targets up to 6kb in length.  PCR products contains a A-tail at the 3’ end and can be used in TA cloning.

The enzyme is stable at room temperature for 4 weeks without affecting the product performance.

 

Use restrictions

For research use only.  Not for use in diagnostic procedures.

Applications

  • Routine PCR
  • High throughput screening
  • TA-cloning
  • Genotyping
  • Library construction
  • Methylated DNA
  • Crude extract PCR

Specification

Detail
Format Enzyme and buffer separate
Polymerase concentration 5U/µl
Buffer concentration 5x
Direct gel-loading No
Error rate 1 error per 2.0 x 105 nucleotides incorporated
Increased specificity No hot-start modification
Fidelity (vs. Taq DNA polymerase) 1x
Proofreading activity No
Exonuclease activities 5’-3’ exonuclease activities
Amplicon range Up to 6kb
Product 3’ ends A-tailed
Shelf life 12 months (if stored correctly)

Ordering info

Cat no. CM2211-0500 CM2211-1000
Pack size 500 units 1000 units
CelTaq DNA polymerase, 5U/µl 1 x 0.1ml 2 x 0.1ml
CelTaq buffer, 5x 4 x 1ml 8 x 1ml

Documents

CelTaq DNA Polymerase Product Brochure

CelTaq DNA Polymerase Product Manual

CelTaq DNA Polymerase Product Manual – mobile

CelTaq DNA Polymerase MSDS

Storage

Store the kit at -20°C upon arrival.  This product is shipped on ice blocks and can be kept at 4°C for 4 weeks without affecting the product performance.  Full enzyme activity is retained for 12 months when stored at the recommended conditions and can endure up to 30 freeze/thaw cycles.

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