CelTaq HS DNA Polymerase

Hot start DNA Polymerase with enhanced specificity
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Cat no. CM2311 Category Features ,

Highlights

  • Reduced non-specific amplification and primer dimers
  • Room temperature reaction set-up with increased yield and specificity
  • Efficient amplification of up to 6kb amplicons
  • Balanced salt solution with proprietary enhancers and stabilizers eliminates the need for buffer optimization
  • Superior performance on high GC/AT templates

Description

CelTaq HS DNA Polymerase is a reliable, cost-effective solution for PCR applications that is subject to long reaction set-up times.  The polymerase utilizes advanced antibody technology to block enzyme activity at room temperature, eliminating the production of non-specific products and the formation of primer dimers.  The polymerase is activated by denaturing the antibody at 95°C before cycling commences, leading to significantly enhanced assay specificity and an increase in amplicon yield.

This robust enzyme tolerates PCR inhibitors and can effectively amplify targets directly from bacterial culture, urine and blood samples. The system produces high yields under standard and fast cycling conditions to effectively amplify targets present in low copy numbers.

The optimised 5x buffer system (containing dNTPs, MgCl2) comprises of a balanced salt solution, supplemented with enhancers and stabilizers enabling consistent, reliable amplification of difficult templates, rich in GC/AT content and does not require additional optimization.

The enzyme is stable at room temperature for 4 weeks and is extensively purified via chemical, physical and enzymatic removal of host DNA to yield ultra-low background.

 

Use restrictions

For research use only.  Not for use in diagnostic procedures.

Applications

  • Difficult templates
  • Routine PCR and multiplexing
  • High throughput screening with extended reaction set-up
  • Low copy template detection
  • TA-cloning
  • Genotyping
  • Colony PCR
  • Direct PCR (urine, blood, bacterial cultures)

Specification

Detail
Format Enzyme and buffer separate
Polymerase concentration 5U/µl
Buffer concentration 5x
Direct gel-loading No
Error rate 1 error per 2.0 x 105 nucleotides incorporated
Increased specificity Antibody-mediated hot start
Fidelity (vs. Taq DNA polymerase) 1x
Proofreading activity No
Exonuclease activities 5’-3’ exonuclease activities
Amplicon range Up to 6kb
Product 3’ ends A-tailed
Shelf life 12 months (if stored correctly)

Ordering Info

Cat no. CM2311-0250 CM2311-1000
Pack size 250 units 1000 units
CelTaq HS DNA polymerase, 5U/µl 1 x 0.05ml 4 x 0.05ml
5x CelTaq HS buffer 2 x 1ml 8 x 1ml

Documents

CelTaq HS DNA Polymerase Product Brochure

CelTaq HS DNA Polymerase Product Manual

CelTaq HS DNA Polymerase Product Manual – mobile

CelTaq HS DNA Polymerase MSDS

Storage

Store the kit at -20°C upon arrival.  This product is shipped on ice blocks and can be kept at 4°C for 4 weeks without affecting the product performance.  Full enzyme activity is retained for 12 months when stored at the recommended conditions and can endure up to 30 freeze/thaw cycles.

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