CelTaq HS DNA Polymerase is a reliable, cost-effective solution for PCR applications that is subject to long reaction set-up times. The polymerase utilizes advanced antibody technology to block enzyme activity at room temperature, eliminating the production of non-specific products and the formation of primer dimers. The polymerase is activated by denaturing the antibody at 95°C before cycling commences, leading to significantly enhanced assay specificity and an increase in amplicon yield.
This robust enzyme tolerates PCR inhibitors and can effectively amplify targets directly from bacterial culture, urine and blood samples. The system produces high yields under standard and fast cycling conditions to effectively amplify targets present in low copy numbers.
The optimised 5x buffer system (containing dNTPs, MgCl2) comprises of a balanced salt solution, supplemented with enhancers and stabilizers enabling consistent, reliable amplification of difficult templates, rich in GC/AT content and does not require additional optimization.
The enzyme is stable at room temperature for 4 weeks and is extensively purified via chemical, physical and enzymatic removal of host DNA to yield ultra-low background.
For research use only. Not for use in diagnostic procedures.